Defendant Eddie Duane Stevey, convicted of various sex crimes against his 16-year-old victim, contends the interpretation of the
Here, the prosecution offered no new science, no breakthrough technology, and no untested kits or tests. No defense expert disputed the efficacy of the interpretive methodologies used at trial; no studies exposed any flaws in the analytical methods used by the county criminalists; and no cases were cited in which the methodologies were discredited within the scientific community. We affirm the trial court's determination that the prosecution did not offer the type of new scientific technique that necessitates a Kelly prong-one evidentiary hearing.
In May 2007 K.E., a 16-year-old junior in high school, lived with her mother, who was in and out of prison for various drug offenses. K.E. was a decent student, played a musical instrument in the school orchestra, was a member of the California Cadet Corps, and tried hard to please her mother. Yet she also stole from her godmother, was afraid of her mother, smoked cigarettes and marijuana, had sexual intercourse with a neighbor boy, Michael, shortly before the crimes alleged here, and lied that she had never had sex with anyone before defendant.
Defendant had lived with K.E. and her mother in 2005 and 2006. K.E. confided in defendant as a good friend, sharing information with him about smoking, drugs, and sex that she would never discuss with her mother. She told defendant she had sex with Michael. Since defendant then lived just down the street, he continued to visit regularly even after moving out of K.E.'s house.
On the evening of Sunday, May 6, defendant was out with his girlfriend and was drinking. His girlfriend complained about his sexual performance. On their way home, defendant got out of the car and began socializing
K.E. testified that defendant knocked on her bedroom window sometime between 10:30 and 11:30 p.m. and asked her to let him in. When she went to the door to the patio, she found him already in the living room. She asked defendant where her mother was and told him he should not be in the house when her mother was not. He followed her into her bedroom and told her, "this is a golden opportunity." He engaged in multiple sex acts over several hours in an apparently futile attempt to get an erection and ejaculate.
According to K.E., he began by pulling down her pajama bottoms and panties and orally copulating her on her bed. He asked her to suck his penis, but she refused. He sucked and licked her breasts before pulling her to the floor. There, he digitally penetrated her vagina while masturbating. He held a candle near her vagina and told her how "good [it] tasted" and how beautiful her "pussy" was. Sporadically, he would penetrate her vagina with his penis, but because he could not achieve an erection, he would return to masturbation. At some point, he asked K.E. to get him some lotion.
By then K.E. had heard her mother return home, but she was too embarrassed and ashamed to tell her mother what was happening in her bedroom. She retrieved cocoa butter lotion from the living room, may have gone into the bathroom, and returned to her bedroom where, she told the jury, defendant continued to masturbate with the lotion. Although defendant would not allow her to dress or go to bed for several hours, eventually she got into bed and fell asleep. He woke her up to let him crawl through her window. There was a spa with a cover under her window. The police found that the spa cover had disturbed dust on it as if someone had climbed over it.
K.E. went to school the next day without taking a shower or changing her panties. She told two friends at school what had happened. That evening, she told her brother's girlfriend, who immediately told K.E.'s mother; her mother called the police. K.E. went across the street to visit Michael until the police arrived. She gave a lengthy statement to the officers and denied she had ever had sexual intercourse with anyone before defendant penetrated her.
The police took K.E. to the UC Davis Medical Center for a sexual assault examination. A nurse practitioner collected blond hairs from K.E.'s vagina and cervix, four swabs from her vagina, blood and urine samples, and potential saliva from her breasts. Pubic hairs and a buccal swab reference sample were collected from defendant and Michael.
Defendant testified, though his testimony was not entirely beneficial to his case. His account is peppered with vulgarities we have no reason to repeat. Nor was he much of an historian since he does not wear a watch and admitted that he has no sense of time. And while insisting that he is no snitch and did not tell the police that K.E.'s brother and his friends had beaten and seriously injured him, he volunteered to the police that his son used and sold drugs. Realizing his testimony and behavior were not helpful, he apologized to the judge for his behavior in court.
Nevertheless, defendant insisted that he did not, and would not, have any sexual contact with a minor. He admitted he was under the influence of drugs and alcohol and could not remember everything, but he knew he would never have sex with a minor because he had been molested by a babysitter when he was a boy.
Defendant denied going back to K.E.'s house at all. He claimed she fabricated the allegations because he had discovered her with Michael in the carport near a "1900 fire stove, wood stove," and he had threatened to tell her mother. Defendant had overheard K.E. telling defendant's son that she had lost her virginity to Michael, someone defendant did not like. He testified that after talking to K.E., he went home and did not go out again that evening.
DNA testing results in a genetic profile of a person, and when the DNA profile of a crime suspect matches the DNA profile derived from the crime scene, criminalists calculate the probability that anyone else's DNA profile would have matched the DNA profile from the crime scene sample. Several criminalists employed by the Sacramento County District Attorney's Crime Lab (county crime lab) testified for the prosecution about the collection, methodology, and interpretation of the DNA testing done on the samples collected from K.E., Michael, and defendant. Some of the results of the testing benefitted the defense; others corroborated K.E.'s account of what defendant had done to her.
The DNA testing of the sperm fraction taken from K.E.'s panties excluded defendant and was consistent with K.E.'s sexual partner, Michael. The DNA results from the labia swab showed a full profile consistent only with K.E.
We begin with the testimony regarding the pubic hairs. Preliminarily we note that it did not take sophisticated DNA testing to confirm that the pubic hairs were blond. Defendant's pubic hairs were blond; neither Michael's nor K.E.'s were.
Prosecution criminalist Angelynn Shaw performed STR (short tandem repeat) and Y-STR DNA analysis on the pubic hairs. Y-STR's are found only on the Y chromosome; thus, they only appear in males. Because the Y-STR testing ignores the female DNA that often overwhelms the male DNA, it is a helpful method when the sample contains a mixture of both male and female DNA. Unlike other kinds of DNA testing, however, it is not possible to identify a particular individual because a male inherits the DNA type from his father and shares the same type with male siblings, uncles, and cousins. According to Shaw, Y-STR testing is generally accepted in the scientific community and is commonly done in labs in the United States and internationally. Y-STR analysis involves the same instruments, the same software, and the same methodology as STR DNA analysis, including isolation (also called extraction), quantitation, amplification, and typing.
While the scientific testing is the same in STR and Y-STR analyses, the interpretation of the value of the results based on the probability of a match differs. Experts employ various statistical methods, depending in part on whether the individual alleles within each locus are statistically independent from one another. With STR results, an analyst uses the "product rule," whereby the frequencies with which each measured allele appears at each tested locus are multiplied together "`to generate a probability statistic reflecting the overall frequency of the complete multilocus profile.'" (People v. Nelson (2008) 43 Cal.4th 1242, 1259 [78 Cal.Rptr.3d 69, 185 P.3d 49] (Nelson), quoting People v. Soto (1999) 21 Cal.4th 512, 525 [88 Cal.Rptr.2d 34, 981 P.2d 958] (Soto).) The product rule cannot be used to calculate the probability of a match with the results of a Y-STR analysis, however, because the traits are not inherited independently. Thus, Shaw testified that she used a simple and well-accepted counting method to determine the frequency of the profile.
Shaw performed STR analysis using the Identifiler kit and Y-STR analysis using the Yfiler kit on three pubic hairs. She was able to obtain a complete profile using the Yfiler but was only able to obtain allele calls at a few locations using the Identifiler because there was more female DNA present than male. Applying the product rule to the STR analysis, the random match probability was one in 562 for Caucasians. The profile produced from the Yfiler was the same as defendant's sample Y profile. Using the counting method and applying the confidence interval, the probability increased to one in 539 individuals.
Jeffrey Herbert, a criminalist from the county crime lab, explained the DNA testing and results obtained from the right breast swab. He provided a basic primer on DNA testing for the jury, noting the four steps in the DNA analysis process: extraction, quantitation, amplification, and typing. In the first step, DNA is extracted from cells. In cases where female and male DNA are mixed in the sample, chemicals are used to separate the two. Once the quantity of DNA is determined, select regions of the DNA, referred to as genetic markers, are amplified to make more copies of the DNA for analysis. The markers targeted for forensic analysis are called short tandem repeats (STR's). These are locations (loci) where a short segment of the genetic code repeats itself. The number of repeats at specified locations varies among individuals and becomes the basis for distinguishing between them. Each person's DNA contains two copies of these markers — one copy inherited from the father and one from the mother. Variations between them in the number of repeats are referred to as alleles. Thus, for example, an STR marker inherited from the mother might have 10 repeats while the marker inherited from the father has 15; each marker is an allele. Fifteen locations are targeted for amplification.
Following amplification, the last phase of DNA testing — typing — takes place. Fluorescent tags are added to the DNA. The sample is run through an instrument that produces an electropherogram. An electropherogram produces
Labs set protocols to insure the integrity of the DNA profile. Herbert explained that in a sample there are real peaks representing alleles, but there may also be noise from the electricity used in producing the electropherogram and artifacts, that is, miscellaneous matter that is not DNA. If, therefore, the height of a peak is below 75 RFU, the protocol of the county crime lab is to not identify the peak as an allele. The peaks below 75 RFU might represent a very low amount of DNA, or they might represent noise or artifacts.
The swab taken from K.E.'s right breast was from a single source, but it did not produce a complete profile because there were a number of markers below the 75 RFU threshold. The sample may have degraded or some of the markers may have been inhibited by external factors. As a consequence, Herbert testified the partial profile was of 10 out of 15 loci. All 10 of the alleles were the same as those of defendant. Some of the 10 were also shared by K.E. or Michael or both.
Herbert determined that the partial profile occurred at random among unrelated people at the probability of one in 100 billion in the Caucasian population, one in six trillion in the African-American population, and one in 450 billion in the Hispanic population. The prosecutor asked Herbert to recalculate the probability by subtracting the alleles that defendant shared with K.E. and/or Michael. By excluding the shared alleles, the probability that anyone but defendant contributed the DNA was one in 12,000 in the Caucasian population, one in 7,200 in the African-American population, and one in 23,000 in the Hispanic population.
Defendant moved to exclude any testimony involving the interpretation of peak heights produced by the Identifiler kit and the interpretation of mixtures by subtracting genetic profiles based on peak height. After the hearing, the trial court denied the defense motion. Defendant enlarges the scope of his challenges to the admissibility of the DNA evidence on appeal. He contends the trial court erred by failing to have an evidentiary hearing to establish that the interpretations of the DNA test results are generally accepted in the scientific community. (Kelly, supra, 17 Cal.3d 24.) But he overlooks something much more basic — Kelly only applies to new scientific techniques. We
Defendant contends the trial court erred by denying his request for a full evidentiary hearing, under the first Kelly prong, to determine whether the Y-STR testing and the methodology used to interpret the results of the DNA testing on mixed samples were generally accepted in the scientific community. Although the science of mathematics may be complicated, "our function is mercifully simple." (People v. Reilly (1987) 196 Cal.App.3d 1127, 1148 [242 Cal.Rptr. 496] (Reilly).) "With respect to the first prong of this test, `reliability' means that the technique `"must be sufficiently established to have gained general acceptance in the particular field in which it belongs."' (People v. Kelly, supra, 17 Cal.3d at p. 30, italics omitted.) In determining whether there has been `general acceptance,' `[t]he goal is not to decide the actual reliability of the new technique, but simply to determine whether the technique is generally accepted in the relevant scientific community.' [Citation.]" (People v. Morganti (1996) 43 Cal.App.4th 643, 656 [50 Cal.Rptr.2d 837] (Morganti).)
A finding of "general acceptance" presents a mixed question of law and fact. "`[W]e review the trial court's determination with deference to any and all supportable findings of "historical" fact or credibility, and then decide as a matter of law, based on those assumptions, whether there has been general acceptance.' [Citation.]" (Morganti, supra, 43 Cal.App.4th at p. 663.)
Once a scientific technique, including probability calculations, is generally accepted, a defendant challenging the technique must offer new evidence. (Bolden, supra, 29 Cal.4th at p. 546.) For example, the utilization of the
DNA testing is tricky when the criminalist, as here, discovers mixed sources in the sample. The DNA evidence extracted from the pubic hairs from inside K.E.'s vagina was introduced as evidence to corroborate her testimony that defendant had sexual intercourse with her as alleged in count one.
"[T]he use of polymerase chain reaction and short tandem repeats technology to analyze a mixed-source forensic sample is neither a new or novel technique or methodology." (Smith, supra, 107 Cal.App.4th at p. 665; see Hill, supra, 89 Cal.App.4th at p. 57; Venegas, supra, 18 Cal.4th at pp. 57-58, fn. 6; People v. Allen, supra, 72 Cal.App.4th at p. 1097; People v. Wright (1998) 62 Cal.App.4th 31, 34 [72 Cal.Rptr.2d 246].) In the absence of California authority directly on point, it is appropriate for us to turn to relevant decisions from other jurisdictions to determine whether Y-STR has achieved consensus
Recent cases in New Jersey and Washington are particularly helpful in understanding the basic science, the similarity between PCR/STR and Y-STR testing, and the scientific response to Y-STR testing. In Calleia, supra, 997 A.2d 1051, the court explained: "The analytical procedure followed in Y[-]STR DNA testing is identical to that followed in autosomal STR DNA testing. The sample is extracted in the same manner, amplified by the PCR method, tagged with a primer, and detected in the genetic analyzer. The data is collected and represented in exactly the same way. The only procedural distinction is that the primer included in the test kit for Y[-]STR DNA analysis contains markers for the Y[-]STR loci specified by SWGDAM [(a scientific advisory board)]; the primer included in the test kit for autosomal STR DNA analysis contains markers for loci on all twenty-three chromosome pairs. The major difference between autosomal STR DNA analysis and Y[-]STR DNA analysis is in the interpretation and application of the test results." (Id. at pp. 1062-1063.)
The similarity between PCR/STR testing and Y-STR testing was also observed in State v. Bander (2009) 150 Wn.App. 690 [208 P.3d 1242] (Bander). "ReliaGene used a PCR-based process known as YSTR testing to type the DNA samples it tested. YSTR amplification is essentially the same as the PCR-STR process that Frank used, except that it permits the analysis of only male DNA in a mixed-source sample that also contains DNA from a female contributor." (Id. at p. 1246.)
Both the strength and the weakness of the Y-STR testing is the fact that only males have the Y chromosome. (Calleia, supra, 997 A.2d at p. 1063.) As a result, the Y-STR testing can be used to resolve difficult mixed-source samples by examining the DNA of only the male. The court in Calleia further explained Y-STR test's limitations. "Because only males possess Y chromosomes, a mother does not contribute to the genetic code of her son's Y chromosome. The DNA sequence on the Y chromosome is passed in complete form from grandfather, to father, to son and on down the male lineage. The Y chromosome loci are not independent of one another and there is no recombination of DNA. It is strictly a male marker and there is no randomness on the chromosomes. Consequently, the product rule used to generate probabilities for autosomal STR DNA analysis is inapplicable to Y[-]STR DNA analysis. In other words, barring random mutations, all men in a paternal lineage will possess the same Y[-]STR DNA profile. Thus fathers, sons, brothers, uncles, and paternal cousins cannot be distinguished from one another through a Y[-]STR DNA profile. [¶] For this reason, Y[-]STR DNA
The fact that Y-STR DNA testing cannot positively identify an individual does not mean the test is a new technique, that it is unreliable, or that the results are not probative. The Calleia court analogized the results of Y-STR testing to conventional forms of evidence routinely admitted in criminal trials, such as shoe imprint evidence. Evidence of shoe imprints found at a crime scene is routinely admitted, the court observed, to connect a criminal defendant with shoes found in his possession, "despite the fact that any number of persons might own identical pairs of shoes." (Calleia, supra, 997 A.2d at p. 1066.) The prosecution was not required to prove that the defendant's shoes were the only ones that could have made the impressions; rather, the jury was provided the opportunity to weigh their probative value. In the same way, the probative value of the Y-STR is a question of the weight of the evidence, not its admissibility. (Id. at pp. 1066-1067.)
Most importantly for our purposes, it was "established that Y[-]STR DNA analysis is a `non-experimental, demonstrable technique' that is widely accepted by forensic scientists." (Calleia, supra, 997 A.2d at p. 1064.) The court concluded that "there is a general acceptance of Y[-]STR DNA analysis in the scientific community." (Ibid.) Similarly, in Curtis, supra, 205 S.W.3d 656, the appellate court affirmed the trial court's finding that "the YSTR methodology had been validated `internally and externally' and subjected to peer review, that it was generally accepted in the scientific community, and that the YSTR evidence was reliable and relevant." (Id. at p. 661.)
Defendant cites to no cases in which the reliability of Y-STR testing has been challenged or questioned by members of the scientific community. Nor has he cited any scientific study that questions the validity or the reliability of Y-STR analysis. Belatedly pointing to National Research Council, The Evaluation of Forensic DNA Evidence (1996), he argues that each new PCR system must be individually validated. We will not address his argument in depth for two reasons. First, he forfeited the argument by failing to raise it in the trial court. (People v. Doolin (2009) 45 Cal.4th 390, 448 [87 Cal.Rptr.3d 209, 198 P.3d 11] (Doolin).) Second, he has not demonstrated that Y-STR is a new PCR system.
Defendant also belatedly claims the trial court erred by failing to establish that the criminalist used the proper procedures in selecting too small a database in calculating the probability of a match. He forfeited this particular argument by failing to raise it in the trial court. (Doolin, supra, 45 Cal.4th at p. 448; People v. Coleman (1988) 46 Cal.3d 749, 776-777 [251 Cal.Rptr. 83, 759 P.2d 1260].) Moreover, we find the trial court did not abuse its discretion by relying on Shaw's testimony that she followed correct scientific procedures, that is, that she counted the number of matches within the U.S. Y-STR
The court allowed a second criminalist to testify about the results of the DNA testing of the sample of saliva taken from K.E.'s breast to corroborate K.E.'s testimony that defendant had licked her breast as alleged in count four. Defendant does not contend that the PCR/STR test procedure is a new scientific technique, as that methodology has met the Kelly threshold in this state for years. What he does argue is that the interpretation of those results, relying on RFU levels that are not standardized throughout the country, is a new scientific technique that should have been subjected to a Kelly prong-one hearing. We disagree and will explain in three steps: presenting the scientific context, debunking the assumption there must be national standards, and illustrating how the methodology used in this case is not a new scientific technique but an application of a widely used and accepted model.
Defendant, however, challenges the manner in which the criminalist interpreted the results. The county crime lab, as pointed out in the statement of facts, has a protocol requiring a peak height measuring at least 75 RFU's to be identified as an allele and thereby to be used to construct a DNA profile. Thus, the peaks below 75 RFU's were not considered even though, defendant argues, they may have represented DNA matching either Michael or K.E. In essence, defendant contends the lab's artificially high RFU threshold established by the lab's protocol may have excluded exonerating evidence from the jury's consideration. But in any event, defendant maintains the court should have held an evidentiary hearing whereby the prosecution would have had to prove that the criminalist's methodology was generally accepted because his reading of the peaks represents a new scientific technique subject to a Kelly threshold showing.
The same is true here. The criminalist testified to no new revolution in science or in probability analysis. Defendant fails, therefore, to meet the very threshold showing that the prosecution is relying on a new scientific technique. Appropriately, the defense seized on the opportunity to vigorously cross-examine the criminalist's methodology and the interpretation of the results. As a result, the jury heard all the evidence and argument as to why dropping peaks below 75 RFU unfairly disfavored defendant and why excluding shared alleles cast doubt on the strength of the resulting profile. Thus, the jurors found themselves in the same shoes worn by jurors in all technical and scientific cases wherein they are asked to determine the weight of the expert testimony in light of its inherent weaknesses or flaws and the specific methodology utilized by the expert in the case before them.
The judgment is affirmed.
Nicholson, J., and Hull, J., concurred.
